Identificación de microorganismos de suelo de páramo alto andino potencialmente electrogénicos para producir bioelectricidad.

Abstract

The present investigation identified potential microorganisms, bioelectricity generators in microbial fuel cells (MFC), was monitored for 16 days in a data acquisition system (DAQ), using organic waste substrates, fruits and vegetables (ratio 50:50 ) and as an inoculum high Andean moor soil, at two depths (20 and 40cm) with 3 replications of each treatment. The presence of biofilm in carbon fiber tissue was checked by scanning electron microscope. The alternative hypothesis was proven to be accepted and it is concluded that at higher depths higher voltages occur: 0.3384 V, 0.2839 V, 0.2112 V; and at 20cm: 0.2539V, 0.2088V, 0.1625V. Genetic analysis by means of massive sequencing by taxonomic profiles concluded that the most abundant bacterial strain of the soil is Bradyrhizobium canariense, which appears to a lesser extent in the biofilm and Geobacter psychrophilus, identified as potentially electrogenic, so it is assumed that they are generators of bioelectricity. n the taxonomic profile of the membrane species predominate; Pseudomonas azotoformans Gluconobacter cerinus, Leuconostoc citreum, Acetobacter okinawensis. Biochemical tests of Urea Agar, Mannitol Agar, Citrate, TSI tests, and SIM Agar for mobility and Indol were used for microbial identification, which includes Pseudomonas aureginosa, agreeing in gender with Pseudomonas flavescens and Pseudomonas pseudoalcaligenes; indicating the correlation between species in the different molecular tests. It is concluded that at a greater depth of the soil of the high Andean moorland at 4000 m.a.s.l. and in pajonal vegetation cover there is a greater amount of electro genic microorganisms. So it would be recommended to investigate other factors such as types of substrate for food, such as sewage, or synthetic residues, in addition to implementing other types of microbial identification tests.