Diseño de un proceso para la obtención de una crema antibiotica a partir del extracto de las hojas de Churiyuyo (Kalanchoe pinnata).

Abstract

The objective was to design a process for obtaining an antibiotic cream from the extract of Churiyuyo leaves (Kalanchoe pinnata) for antibiotic use in superficial skin infections. The fresh leaves of the Chiriyuyo plant were used as raw material to then be crushed and thus be able to break the cell walls and obtain an active extract, then through maceration in an alcoholic environment in this case methanol on a 60%. With which we proceed to make a filtration that will facilitate the concentration of the extract in a rotary evaporator. Laboratory scale tests were performed in order to obtain the best result in bacterial inhibition to the 5% and 10% in both as in the extract and the cream, giving the following results for the antibiogram test for the strains: Staphylococcus aureus 1.25 cm of halo of inhibition, escherichia coli 1.6 cm of halo of inhibition, klebsiella pneumoniae 1.1 cm of halo of inhibition. In the thermo-resistance tests in the quality control it gave us in the organoleptic Factors the whitish yellowish color, in the viscous solid aspect, in the characteristic smell, in the Physicochemical Factors the pH gave us 6.5. In the extensibility test it complies with the specified parameters of the technical standards regulations INEN 2867. In the microbiological test the cream had ABSENCE in total coliforms, 20 CFU / mL in aerobis mesophiles and ABSENCE in molds and yeasts. With these results it was possible to design the process to obtain the cream from Churiyuyo leaf extract, checking the antimicrobial activity against different bacterial strains and also meets all the parameters established by the technical standards regulations INEN 2867 for cosmetic products, it is recommended to perform additional studies to isolate specific components to increase efficiency process.