Determinación de las actividades fotoprotectora, genotóxica y de irritación ocular in vitro del extracto hidroalcohólico de Lippia triphylla.

Abstract

The purpose of the present research work was to determine the photoprotective, genotoxic and ocular irritation activity of Lippia triphylla. hydroalcoholic extract. The conditioning and quality control of the crude drug and the respective phytochemical screening were carried out, where the presence of phenolic compounds and flavonoids was demonstrated. Using spectrophotometric methods, the Sun Protection Factor could be determined with a result of 2 for Lippia triphylla extract, a SPF of 13 for ethylhexyl methoxycinnamate (OMC) and a SPF of 18 for octyldimethylPABA. 20 ppm concentration. The photoprotective activity evaluated in vitro through the method of cell death induced on bacteria E. coli ATCC 25922 showed that at high concentrations the protection factor with respect to UVB radiation increases due to the longer survival of the bacterial colonies; with respect to the concentration of 2000 ppm of the extract of Lippia triphylla it showed a high protection to 90 minutes, for the OMC and PABA standards the surviving UFC were up to120 minutes. In relation to the amount of phenols and flavonoids present in the extract, the results were 4.864 mg Equivalents to Quercetin/g lyophilized for total flavonoids and 16.48 mg Equivalents to Gallic Acid/g lyophilized for total phenols. The free radical uptake capacity obtained an LC50 of 709.66 (ug mL -1). Toxicological studies were performed based on ocular irritability and genotoxicity respectively, with alternative tests to Draize test such as HET-CAM and CAM -TBS with a result of No Irritants for both cases. Finally, to determine the degree of genotoxicity, the roots of Vicia faba were used, where the different extracts of Lippia triphylla did not cause alterations in the cell division or the formation of micronuclei, it means, they did not affect the DNA or proteins that regulate mitosis.