Síntesis, caracterización y funcionalización de nano-partículas magnéticas en grafeno oxidado visando aplicaciones en terapia fotodinámica.

Abstract

The objective of this thesis was to study the temporal stability of the nanocomposite GO-NPFes-Mentil ZnPc-VCLPEGDA aiming applications in Photodynamic Therapy (PDT). For this, the co-precipitation method was used to synthesize the NPFes, setting the synthesis parameters such as the pH, the concentration of the iron salts involved (Iron ll and Iron lll) and the temperature. The synthesis process of the VCL PEGDA nanogel was obtained by the polymerization of an emulsion (SDS) in a continuous reactor (Nitrogen) using a cationic initiator (Ammonium Persulfate). The chemical identification of the nanocomposite was obtained by Raman characterization, which allowed c rroborating the existence of nanoparticulate material (hematite and reheated magnetite) and of the GO by its D and G bands at approximately 1350 cm-1 and 1600 cm -1 respectively. The electronic configurations and structural distributions of the nanocomposite were obtained by FTIR characterization, observing asymmetric and carboxylic C-O, H-O, C = O bonds and Fe-O iron bonds. The chemical and physical properties, as well as the dimensions of the nanocomposite were evidenced by AFM characterization, whose results show GO sheets of 1,023 micrometer long, with NPFes whose average thickness is 30,105 nanometer, and a standard deviation of 1.15, which is a present error due to the aggregation between NPFes and possible impurities. Finally, a UV / Vis spectroscopic study was carried out to analyze the saturation and evolution of the absorbance over time of the nanocomposite with 68% absorbance intensity after 2 days of study in relation to the first minutes of synthesis. . The nanocomposite was obtained as a nano-vehicle in Photodynamic Therapy (PDT), since it makes it an excellent biosensor and drug transporter in the future.