Análisis microbiológico de la vertiente del Santuario de Nuestra Señora de la Fuente del Carmelo del barrio Catequilla perteneciente al cantón Chambo provincia de Chimborazo.

Abstract

The objective consisted of a microbilogical analysis of the springof the Santuario de Nuestra Señora de la Fuente del Carmelo, of the Catequilla neighborhood, belonging to the Chambo canton, Chimborazo province. The investigation development was carried out in the clinic analysis anda microbiological labs of the Science Faculty of the Escuela Superior Politécnica de Chimborazo, in which the different tests were carried out for the quantification and identification of the mesophyll aerobic baceteria E.coli/Coliforms, <Staphylococcus, molds and yeasts. The quantification of the microorganisms was developed using the Petrifilm plate method; the colonies were isolated through mincing in Mueller-Hinton agar; later, the analysis and macroscopic description, Gram dyeing, biochemical testing, oxidase, catalase, antibiogram were carried out, including confirmatory testing for positive and negative Gram bacteria to each isolated colony; 21 bacteria strains were isolated and identified which correspond to the morphological types og Gram positive Cocci (62%)and Gram negative Bacilli (38%) : This shows that in the spring wáter there is a prevalence of the Gram positive Cocci. The found species were Staphylococcus aureus (29%), noting the presence of native bacteria and other found in the soil and wáter from other sites. On the 21 identified clones the antibiogram was carried out by the diffusion in agar method, observing that most of the isolated bacteria were resistant to the used antibiotics. The Chambo Canton Municipality, together with the water analysis lab, is recommended to carry out maintenance and follow up of the piping so as to avoid posible contamination wich can be produced by this factor.