Evaluación de la actividad inmunomoduladora del extracto acuoso de Pleurotus ostreatus sobre Rattus norvegicus tratados con ciclofosfamida.

Abstract

The present investigation aimed to evaluate the immunomodulatory activity of an aqueous extract of Pleurotus ostreatus on Rattus norvegicus treated with cyclophosphamide. The first phase consisted of the extraction of an aqueous extract of polysaccharides from the Pleurotus ostreatus fungus using a digestion process (maceration at high temperatures) as the extraction method. The first phase was the evaluation of immunomodulatory activity by an experimental test in 15 female Winstar rats (Rattus norvegicus), divided into three study groups (white, high and low concentration), and the extract was given to different concentrations according to the study group (O, 50 and 200mg / kg) intraperitoneally for seven days, with the administration of a single dose of cyclophosphamide orally on the filth day of the study to the low and high concentration groups. The third phase consisted in the evaluation of the effects produced by the administration of the extract in the organism of the study subjects by means of the accomplishment of blood biometrics before and after the administration of the extract. As a result of this study it was obtained that administration of the extract mainly affects the levels of two types of cells, monocytes and granulocytes, which show increased levels and related directly proportional to the concentration of extract administered after treatment. It was concluded that the use of the aqueous extract of Pleurotus ostreatus produces a modification of the cells of the immune system (monocytes and granulocytes), cells of great importance in the immune response of the organism, mainly to infection processes. It is recommended to avoid prolonging the actions that cause unnecessary stress to the specimen, thus facilitating the work performed.