Aislamiento de microorganismos probióticos del tracto intestinal de Gallus gallus en tres estadios fisiológicos de pollos

Abstract

In the LAB of the faculty of Animal Science, it was isolated the microorganisms of the intestinal tract (colon and cecum), of chickens in three physiological stages: initial, growth and fattening, with the purpose of obtaining probiotic strains, such as that can be used in the same species. The experiment lasted 120 days, the experimental units were distributed in a system of randomization of each one of the digestives tracts of the chickens, same that were analyzed under the statistical method, Chi Square, were isolated and identified the probiotic microorganisms that belonging to the genus Lactobacillus, using the methodology based on criteria of identification by morphological characterization, biochemical tests and virulence. The main results that were obtained with the morphological characteristics of the colonies were homogeneous, regular, convex and creamy appearance, the 72 studied samples, in the single morphology, bacilli and cocci were observed. Gram positives except two and three experimental units in the initial stage and growth respectively. The strains were subjected to biochemical tests as catalase, heartburn, gas production, production of H2S and mobility, obtaining favorable results, since the majority presented the characteristics of gender Lactobacillus, acid production from glucose are usually positive except for some species catalase, H2S gas production and negative mobility in most samples of all 24 crops, they presented hemolytic activity and all crops of the genus Lactobacillus, have the capacity to milk clotting. Concluding that the intestinal tract of Gallus gallus, is an important natural source for the isolation of Lactobacillus with probiotic characteristics.