Determinación del perfil de resistencia/susceptibilidad antimicrobiana en cepas de bacterias del grupo de enterobacterias aisladas en queso fresco artesanal elaborados en zonas rurales del cantón Riobamba.

Abstract

The study of resistance/susceptibility in strains of enterobacteriaceae isolated from fresh cheese produced using traditional methods in rural areas of Riobamba canton, it aims to determine the profile of resistance / susceptibility to antimicrobials in strains of bacteria Enterobacteriaceae group. From the eight rural villages of canton Riobamba were chosen three of the most populous and the largest geographic area, San Juan, Quimiag and Pungalá. The collected samples were seeded in violet red bile agar with glucose to deep seeding, the colonies were quantified obteinig as a result in counting 8.86 x 10 3 CFU/g, a 9.88 x 4 CFU/g, finding they were outside of established ranges established by NTE INEN 1528:2012. The colonies were isolated on MacConkey eosin methylene blue agar, obtaining 53 strains of Enterobacteriaceae: Enterobacter cloacae 30%, 21% E. coli and Klebsiella oxytoca, Enterobacter gergoviae 8%, Shigella sonnei 7%, Klebsiella pneumoniae 5%, Proteus mirabilis and Enterobacter aerogenes 4%. The colonies are identified by gram staining, preliminary evidence oxidase and catalase, the genus and species identification was performed through biochemical tests, Kligler, citrate, SIM, LIA and urea. For susceptibility testing a method of distributing Kriby-bauer with the following antimicrobials is done: Ampicillin, amoxicillin/ clavulanate, cefotaxime, ceftriaxone, cefoxitin, imipenem, and ciprofloxacina. It is concluded that 100% of the isolate strains were sensitive to cefotaximina (CTX) and ciprofloxacin (CAZ), 92% to imipenem (IMP), 85% to cefoxitin (FOX), 83% to ceftriaxone (CRO), 53% to amoxicillin/ clavulanate and 10% for ampicillin, this 10% sensitive to ampiciline enterobacteriaceae corresponds to strains of E. coli.