Estudio microbiológico de las aguas termales de Guayllabamba o Aguallanchí situadas en el cantón Chambo, provincia de Chimborazo.

Abstract

A microbiological study was performed on samples of mineral water collected in emerging place located in the thermal waters of Guayllabamba in Chambo, Province of Chimborazo, Ecuador, in order to determine its microbiological quality and get in its native microbiota. A physical and chemical analysis was performed for determining temperature, pH, total dissolved solids and conductivity, microbiological analysis were also performed using the technique about sowing in Petriflim plates, later the bacteria purification were performed through an insulated for obtaining pure bacteria all the purified bacteria underwent by the Gram stain technique for classification in positive or negative, and, accordingly perform the corresponding biochemical tests for each group, finally unidentified bacteria even after biochemical tests were analyzed by a method of identification called System MicrogenTM GN-ID, by this way was found in these waters the existence in a big proportion of Gram negative bacilli bacteria, and low bacilli and Gram positive coccus and also the presence of gender Methylobacterium (14%), Moraxella (14%), Bacillus (29%), and Ralstonia pickettii (29%) and Staphylococcus aureus (14%) species. This study concluded that the thermal waters have poor native microbiota with more of Gram negative microorganisms, with the gender and species Ralstonia pickettii, Methylobacterium spp, and Moraxella spp, the first registered in thermal waters of Guayllabamba in Ecuador studies. It should be noted that the number of strain bacterial isolated in the research, is not a big health danger for people who comes to this hot spring resort. It recommended to local authorities perform deep analysis of the tools used for cleaning these pools to find the small pollution in this water; ensuring the welfare of community and whole people health.