Activdad gastroprotectora de los extractos metanólico, hexánico, y de acetato de etilo de Oreocallis grandiflora sobre ratones mediante el modelo de úlcera inducida por etanol/hcl.

Abstract

Gastro protective activity of methanol extracts, hexane and ethyl acetate cucharila (Oreocallis grandiflora) was assessed using the model of gastric ulcer induced by ethanol/HCl in mi ce ( Mus musculus ) males from the National Research Institute Public Health Bioterio (NRIPH) in Guayaquil. By applying the treatments administered in randomized blocks were three doses (25 , 50 and 100 mg/kg) of different extracts, positive control of omegaprazol (30 mg/kg) and a negative control that was administered the solution used as a vehicle (polysorbate 80.1% w/v). Extracts were obtained by dynamic maceration (1000 rpm), followed by sonication ( 15 minu tes) with subsequent evaporation under controlled condit ions (100 rpm, 60°C), starting each time 20 g of inflorescences grandiflora Oreocallis plant with 80 ml each solvent extraction. Results demostrating significant efficacy at the macroscopic level, the fact that after application of the test statistical analysis ( ANOVA and DUNNET p<0.05) are significant, with the methanol extract (100% protection of the gastric lesion was observed) implying better results, however not be negligible effects due to hexani cos and ethyl acetate extracts, as percentage protection exhibit superior gastric lesion to 97%. At the microscopic level, the study be a pathologist confirmed the above results. The phytochemical study found the majority presence of flavonoid - like compoun ds, followed by phenolic compounds, triterpenes, quinones and lactones. Research conducted study leads to new perspe ctives on this promising plant species in the scope of life sciences and changing the productive matrix of the country.