Producción de Bacillun thuringiensis, Berliner a Nivel de Laboratorio

Abstract

This research was carried out in the Phytopathology Laboratory of the Faculty of Natural Resources of ESPOCH and its objective was to determine the ideal culture medium and optimal fermentation conditions of Bacillus thuringiensis to determine if it is possible to produce a liquid bioformulation used as a bioinsecticide for biological pest control. We proceeded to isolate and identify colonies from two soil samples from Chambo and Alausi. Fermentations were carried out at flask level in two culture media: one, composed of mineral salts, Bacto-peptone and glucose and the second, formed by mineral salts, glucose, soy flour and tomato puree. Subsequently, every 8 hours, the number of spores was quantified by means of a Neubauer chamber, with the data growth curves were made and their pH was determined. With the application of the statistical treatment, analysis of variance and Tukey's test at 5% significance, it was determined that the ideal culture medium is the second one, since it is more enriched with complex substrates that contain most of the nutrients required by the bacteria for their growth and development. The conditions for fermentation were temperature 26ºC, stirring speed of 200 rpm to ensure an adequate oxygen supply and avoid heat accumulation, as well as the optimum initial pH of 7. It is recommended to test its insecticidal activity on insect pests.