Métodos de Congelación de Embriones Bovinos en Nitrógeno Líquido

Abstract

The research got to several agreements. It also determined that freezing by traditional methods is the only methodology that can be used in a large scale on embryo cryopreservation programs produced in vivo. About vitrification, there is a great diversity of protocols and also a high viability in its results which don´t measure at the end of the gestation, but the capacity of the embryo to preserve its characteristics until checking in the lab. These new techniques have showed they adapt to promissory way. They showed better results regarding to traditional and non-traditional methods. However, they have different procedures with common goals: cell´s osmotic dehydration before storing in liquid nitrogen, and prevent deleterious for toxic chemicals in intercellular freezing. It maintains perfect conditions during several years: besides, on each quicker freezing methods as vitrification and non-traditional the research obtained better results preserving an adequate preservation and good pregnancy rate regarding to the slowest where there´s a lower embryo survival.