Criopreservación de semen ovino de la raza Pelibuey y Blackbelly en la provincia de Pastaza

Abstract

The objective of this research was the cryopreservation of sheep semen of the Pelibuey and Blackbelly breeds in the province of Pastaza. In the development of the research, the treatments were TO (Diluent +0.0 mg CLC), TI (Diluent +0.5 mg CLC), T2 (Diluent+1.0 mg CLC), (Diluent +1.5 mg CLC) and a completely randomized design were applied with a combinatorial arrangement of factors where factor A= race and factor B = dose of CLC (mg/120 million spermatozoa), three replicates (straws) were evaluated per treatment. The statistical techniques analyzed were descriptive statistics for macroscopic variables in fresh semen. Descriptive statistics (mean and standard deviation) were used for the ml and pH of the ejaculate. For the pre- freezing microscopic semen variables (mass motility points, individual motility %, vitality %, sperm concentration million spz/ml, morpho-anomalies %), the mean was used, and for the post- freezing variables, individual motility %, sperm viability %, membrane integrity, morphology %. The data obtained were tabulated with the Infostad program, where the separation of means was used through the Tukey test of <0.05. Among the results obtained in the pre-freezing variables, there were no significant differences, while in the post-freezing variables, there were no significant differences; however, in the variable of membrane integrity %, there were significant statistical differences for Pelibuey and Blackbelly males with values of TO:11.33%; T1:25.67%; T2:27.67%; T3: 18.67% and TO: 18%; T1:38.33%; T2:25.7%; T3:22.33%, respectively. The total cost was 594 98 USD. It is concluded that sheep semen had the best characteristics for freezing. and the addition of post-freezing CLC can improve sperm membrane integrity. However, using different levels of CLC to contrast the results obtained in the following study is recommended.