Evaluación in vivo e in vitro de la Actividad de Nanoplata sobre Microorganismos Oportunistas de la Sarna Sarcóptica en Cavia porcellus

Abstract

An in vivo and in vitro study was conducted to evaluate the efficacy of nanosilver at 5, 10 and 20µg/mL against opportunistic sarcoptic mange microorganisms in guinea pigs (Cavia porcellus). These guinea pigs were naturally infested with sarcoptic mange (Sarcoptes scabiei), and most of them presented moderate and crusty dermatitis with secondary bacterial complications. The isolated bacteria showed in vitro 99.9% inhibition in 40 minutes and at a concentration of 20mg/mL. The in vivo evaluation was performed in 5 groups composed of 10 animals each. Group 0: untreated controls. Group 1, 2 and 3: treated with nanosilver gel at concentrations of 5, 10 and 20µg/mL topically, twice a day for 15 days. Group 4: treated with Ivermectin 500mg/100mL with an interval of 7 days. In the 5 groups, the percentage of live mites obtained by skin scraping of each animal was evaluated. Based on the clinical evolution of the lesions, the percentage of effectiveness in the treated group was determined. The evaluation of the lesions allowed observing the recovery of the skin after the detachment of scabs after 12 days of treatment, also, 100% of the animals of this group recovered completely in 30 days, observing hair growth in the affected parts. The results showed that the nanosilver 20µg/mL has a high bactericidal power. It was a valid and economical alternative in the opportunistic organisms that share sarcoptic mange.