Evaluación de semen fresco y diluido de sementales equinos en la Estación Experimental Tunshi

Abstract

The objective of this research was to evaluate the fresh and diluted semen of equine stallions belonging to the Tunshi Experimental Station, of the Higher Polytechnic School of Chimborazo. A completely randomized design was applied with a combinatorial arrangement of two factors, A factor (semen) and B factor (breed), the tabulated normality and homogeneity were evaluated within the investigation where 3 stallions, each one with 4 years old were used to whom 3 semen extractions were performed in a period of 15 days. Ten straws were packed on each occasion and later the macroscopic and microscopic analysis of semen was performed. In the second semen extraction, it was subjected to a dilution with INRA 96 at room temperature, in the third semen extraction, it was diluted with INRA 96 and refrigerated at 5 degrees Celsius for 48 hours. The results demonstrated the existence of a high sperm mortality in diluted semen (70.6%) followed by fresh semen (65.77%) and frozen semen (15.45%). Sperm viability presents significant differences between treatments, evidencing that frozen semen is affected by thermal shock; the cost of the technology applied in this study was $789.75. It was concluded that the equine semen conservation medium influenced both sperm mortality and viability, in addition the volume of the ejaculate of the stallions was affected by climatic conditions and genetic crossbreeding. It is recommended that the study can be replicated considering the sperm viability per animal to visualize which of the genetic crosses is more viable for equine reproduction.