Caracterización molecular de toxocara canis en perros del albergue criar de la ciudad de riobamba

Abstract

The aim of this work was the molecular characterization of Toxocara canis by means of molecular techniques and the determination of prevalence of parasitosis by means of the application of coproparasitic tests. Fecal samples were obtained from 150 dogs from Criar shelter. The parasite was detected by direct examination and the Willis method. In the results of the prevalence, a total of 75 females presented a parasitosis of 52.82%; on the other hand, 67 males 47.18%, in the age parameter, a group of 74 puppies obtained 52.11% and the group of 68 adults 47.89%, while in the quantity of parasites according to the case of monoparasitosis was 71.83% and polyprasitaized 28.17%. According to species, Toxocara canis 35.75%, followed by Giardia canis 32.40%, Chilomastix sp. 7.82%, Ancylostoma caninum 6.70%, Cystoisospora canis 5.59%, Entamoeba coli 7.26% and Iodamoeba sp. 4.47%. In the type of races, half-caste dogs had the highest prevalence of 94.67%, followed by Husky 2%, German Shepherd and Pitbull 1.33% and finally French 0.67%. Finally in the epidemiological analysis a total of 94.67% of positive cases and 5.33% of negative cases. For the molecular study, the Sanger sequencing and Barcode amplification technique was used, in which primers were selected for the amplification by polymerase chain reaction of the internal transcribed spacer region of the ribonucleic acid in which the result was positive both for the fecal sample and for the egg resuspension sample, presenting a high prevalence rate of parasitosis, it is recommended to implement a parasite control within the shelter.