Evaluación de la bioactividad antagónica de BAL y 3-piridincarboxamida sobre Botrytis Cinerea de fresas

Abstract

Botrytis cinerea infects and deteriorates strawberry crops in the post-harvest. Its control by chemicals promotes the risk of resistance, while consumers demand products without pesticides. This study focused on the antagonistic bioactivity of lactic acid and 3-pyridinecarboxamide bacteria on B. cinerea, from strawberries. The dual culture technique pitted BAL of fermented quinoa grown on MRS agar versus Botrytis spores on malt agar. LAB was cultured at 37 ° C, for 24 and 48 hours and supernatants of these cultures and 3-pyridinecarboxamide were tested. An in vivo trial was performed. Additionally, the ecological factors affecting B. cinerea were studied. According to the results, Botrytis was inhibited in 45.50% by LAB cultured at 37 ° C for 24 hours on Malta agar, in contrast, the LAB in PDA inhibited 32.86%. The 48-hour LAB in Malta agar inhibited 29.22% and in PDA 25.86% and the chemical at 3000 ppm inhibited 24.86%. The supernatants of the cultures did not inhibit the pathogen in any case. The in vivo assay showed an efficiency of 63%. Botrytis in strawberries produced a reduction of vitamin C of 19%, and a reduction of total sugars and minerals, the water content changed from 87.62% healthy strawberries to 89.027% and the pH from 3.51 to 3.91%. It is concluded that the LAB tested inhibits better in vitro Botrytis cinerea in Malta agar at 24 hours. The in vivo assay corroborated this bioactivity.