Determinación de la Actividad Insecticida y/o Anti Alimentario del Aceite Esencial de Tzinsu Tagetes minuta en Drosophila melanogaster.

Abstract

The purpose of this investigation was threefold: to extract Tzinsu (Tagetes minuta) essential oil by means of water vapor dragging distilltion. To verify the insecticide and anti feeding activity over fruit fly (red eyes and dark body) Drosophila melanogaster. To detemmine the minimum effective dosis randomly applied on Drosophila melanogaster vito cultivatins.

Both the insecticide and the anti fecding activity were determined by Tzinsu essential ol which was extracted by means of water vapor dragging distilation considering the following data: Yield 0.36%; density 0:85 g/m; reftaction 1.487; solubiliy 1:67; alcohol volume 96%.

Once the essential oil was extracted the following Drosophila melanogaster percentage concentrations were supplied: 0.5%; 1.25%; 2.5% and 5% which served to evaluate mortality as well as food diminishing for obtaining an anti feeding effect.

An appropriate 5% treatment was established by using Turkey's Test; the lethal dosis mean — (Das) was obtained by lincar regression analysis. The tested adult Mies (Drosophila melanogaster) añer three hours time observation together with the respective essential oil 5% concentrations showed that such concentrations killed 100% of flies because the oil contained Tegetone and Trans Ocimeno, these elements were found through gas chromatography analysis which also established a 71% of DL, the necessary amount to kill 50% of Drosophila melanogaster adult insects.

""This way, it was established that the Tzinsu essential oil presents insecticido activity rather than anti fecding one; therefore, the study provides a feasibility von using bio nsctei"Luego se determino los tratamientos más adecuados mediante la prueba de Tukey al 5% y la Dosis letal media (DL50) se determino mediante el análisis de regresión lineal. Las moscas adultos de Drosophila melanogaster analizadas a las 3 horas con las respectivas concentraciones de aceite esencial presentó que la concentración de 5.0 % mata el 100% de insectos adultos debido a la acción de la trans Tagetona y trans Ocimeno en el aceite. Que se determinaron mediante el análisis de cromatografía de gases. Estableciéndose una DL50 de.71% necesarios para matar el 50% de insectos adultos de D. melanogaster. Estableciéndose que el aceite esencial de Tzinsu presenta una actividad insecticida más no anti alimentaria. Propiciando de este modo un estudio sobre la factibilidad de utilización de bio insecticidas.