Estandarización de los valores de referencia hematológicos y bioquímicos en ratones (Mus musculus) cepa BALB/c del bioterio de la Escuela Superior Politécnica de Chimborazo

Abstract

The objective of the present research was to standardize the hematological parameters (primary and secondary) and biochemicals (glucose, cholesterol, triglycerides, albumin, and total proteins) on Mus musculus strain BALB/c mice. Forty blood samples divided between males and females of body weights between 25g to 30g, bred and reproduced in the ESPOCH Bioterium at a temperature of 22 degrees Celsius, a humidity of 65%, with 12 hour light/dark cycles. In the determinations, methods and techniques were used for human blood, an automated Abaxis equipment was used to process the hemogram VetScan HM5 for veterinary use. In addition to taking a blood smear to count leukocyte differential with Wright and Panoptic stain; for blood chemistry analysis, on the one hand, the semi-automated equipment Rayto RT-1904C was used (total protein and albumin), and on the other, the Cobas c111 automated equipment (glucose, cholesterol, and triglycerides). For analysis and the Statistical data, the Minitab18 software was used. The data were compared using the parametric "t" test of two samples resulting in differences between both sexes in relation to secondary indexes, platelets, glucose, cholesterol, and triglycerides, as well as small differences in relation to bibliographic data. It is concluded that the differences can occur due to environmental factors to which each study was exposed as the method of collection, equipment used, age, temperature, humidity, and sex of the animal. That is why despite being within the normal range. It is necessary to obtain custom values for each Bioterium, with its own environmental conditions and intrinsic characteristics.