Estudio preliminar de grupos funcionales en plasma sanguíneo en los estudiantes de la carrera de Biofísica de la ESPOCH, mediante espectroscopia FT/IR

Abstract

The objective of the present investigation was to study the functional groups of the human plasma, by means of FT/IR spectroscopy in ESPOCH students and with a statistical study of the data obtained. For which blood samples were taken from 54 students of the Biophysics career and subsequently the blood plasma was extracted by centrifugation, 20 µl of plasma was used and a drop was placed in the center of the spectrophotometer crystal, which makes a reading of absorption versus wave number; This measurement was performed twice for each sample. Finally, the team exported the data in TXT format which were treated with ORIGINPRO 2018 version 1. Among the results that were obtained, it was identified that the samples of the 54 individuals are homogeneous. Likewise, peak 1 at 3339.62 cm-1 with a width between 3498.24 and 3150.39 cm-1 that presents the water component and peak 2 at 1635.09 cm-1 with a width between 1668.36 and 1610.51 cm-1 were denoted. It presents the protein component. In conclusion, differences were found between the functional groups of a typical blood plasma in which the protein part, the lipid part and the hydrocarbon part are identified and, conversely, in the blood plasma of the study, only the protein part is found and does not allow evidence a difference between the lipid and hydrocarbon part, while the presence of water is very evident. Therefore, since the functional groups have already been identified in this study. It is recommended to expand the number of data, and change the experimental design, to have a database that can be considered as a typical blood plasma spectrum of Riobamba.