Caracterización de mecanismos biocontroladores de Trichoderma spp y evaluación del efecto protector en aliso (Alnus acuminata Kunth) en vivero.

Abstract

The research proposed to isolate pathogens from radicular samples of aliso plant (Alnus acuminata Kunth) which indicated fungal attack symptoms, also to Isolate Trichoderma strains to determine its blocontroller effect. The collection was carried out in 5 forest nurseries in the province of Chimborazo, the inclusion of tissue fragments in PDA medium was the methodology used for the Isolation of phytopathogenic fungi and Trichoderma spp strains, Cultural, morphological and molecular identification were necessary to determine to which gender and specie each of the specified ones corresponds. The strains were preserved by the cryopreservation method in 2 ml ependorf tubes with a 70% glycerol suspension at -20 Cunder refrigeration. From a total of 25 Isolates, 8 strains corresponding to Fusarium soloni 6 strains of Fusarium oxysporum, 1 strain of Fusarium equisete, 1 strain of Fusarium floccferum, 3 strains of Trichoderma harzianum and 1 strain of Trichoderma hamatum, 1 strain of Clonastachys sp,. 1 strain of Dactylonectria novezelandica, 1 strain of Hypocrea lixil, 1 strain of ilionectria panacis and an undetermined strain, were obtained, The Trichoderma harzianum got the highest radial growth rate in the tests with 0,65 mm/h while Hionectria panacis with 0.04 mm/h, with the lowest growth rate recorded. The percentage of growth Inhibition of Trichodenna harzianum against Fusorium solani was 5.6% while compared to Fusorium oxysporum it was 25.5%