Comparación de tres dilutores en la crio - conservación y viabilidad espermática de semen caprino en la estación experimental tunshi.

Abstract

Three diluents were compared in the cryopreservation and sperm viability of goat semen, evaluating the microscopic characteristics of the sperm after the thawing and determining the cost of production of each treatment; this investigation was conducted in the Ovine Caprina and Camelidae Academic Research Unit in Tunshi Experimental Station located in Licto Parish, Riobamba canton, Chimborazo province , with an experimental methodology, under a Completely Random Design for which three treatments with ten repetitions were used, The experimental unit consisted of 1 seminal dose of 0,5 ml frozen in liquid nitrogen, from the 4 year old Saanen goat, obtaining a total of 30 doses under study, with a duration of 120 days of research. Microscopic analysis to post thaw straws were performed at the Laboratory of Reproduction and Artificial Insemination, Faculty of Animal Science ESPOCH. We determined highly significant differences to the means of the treatments applied (P <0,01), for the characteristics of mass and individual mobility in the process of seminal cryopreservation, in terms of sperm viability, no significant differences were recorded (P> 0,05). Obtaining the best results for post thawing when using the TrisYema + Glycerol treatment, registering mass motility (77%), individual motility (2,82 pts.) And sperm viability (83%), however Triladyl treatment, record the following mass motility results (76%), individual motility (2,81 pts.) and sperm viability (88%), there being only numerical differences between the two treatments. A cost of production of 1,36 USD was established, per seminal dose in the three treatments. Concluding that in terms of microscopic characteristics, the best treatments were D1T1L (TrisYema + Glycerol) and D2T2T (Triladyl), recommending the use in the cryopreservation and sperm viability of goat semen TrisYema + Glycerol or Triladyl diluents, for presenting satisfactory results in the parameters studied in the present investigation.